Journal: Journal of microbiology and biotechnology

Article Title: Inhibition of Pathogenic Bacteria and Fungi by Natural Phenoxazinone from Octopus Ommochrome Pigments.

doi: 10.4014/jmb.2206.06043

Figure Lengend Snippet: Fig. 3. Epifluorescence (10x) images of E. coli and S. aureus stained with calcein-AM and propidium iodide (red fluorescence) Live/Dead Kit under treatment with ommochromes (10-0.62 mg/ml).

Article Snippet: For the antibacterial evaluation, the gram-negative pathogenic bacteria Escherichia coli (ATCC 25922), Salmonella Typhimurium (ATCC 14028), Pseudomonas aeruginosa (ATCC 9027), Pseudomonas fluorescens (ATCC 13525) and gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Bacillus subtilis (ATCC 6633), August 2022⎪Vol.

Techniques: Staining, Fluorescence

Journal: Journal of microbiology and biotechnology

Article Title: Inhibition of Pathogenic Bacteria and Fungi by Natural Phenoxazinone from Octopus Ommochrome Pigments.

doi: 10.4014/jmb.2206.06043

Figure Lengend Snippet: Fig. 4. Epifluorescence images (at 10x) of (A) S. aureus, (B) B. subtilis, (C) E. coli, (D) S. Typhimurium, and (E) C. albicans treated with ommochromes and stained with fluorochrome from ROS Kit.

Article Snippet: For the antibacterial evaluation, the gram-negative pathogenic bacteria Escherichia coli (ATCC 25922), Salmonella Typhimurium (ATCC 14028), Pseudomonas aeruginosa (ATCC 9027), Pseudomonas fluorescens (ATCC 13525) and gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Bacillus subtilis (ATCC 6633), August 2022⎪Vol.

Techniques: Staining

Journal: Journal of microbiology and biotechnology

Article Title: Inhibition of Pathogenic Bacteria and Fungi by Natural Phenoxazinone from Octopus Ommochrome Pigments.

doi: 10.4014/jmb.2206.06043

Figure Lengend Snippet: Fig. 6. Microphotographs (10X) obtained in a confocal microscope of microorganisms treated with ommochromes (10 mg/ml) and stained with fluorochrome calcein-modified (row 1) and propidium iodide (row 2) and overlapping both fluorophores (row 3). (A) S. aureus, (B) B. subtilis, (C) E. coli, (D) S. Typhimurium, (E) P. aeruginosa, (F) P. fluorescens, and (G) C. albicans.

Article Snippet: For the antibacterial evaluation, the gram-negative pathogenic bacteria Escherichia coli (ATCC 25922), Salmonella Typhimurium (ATCC 14028), Pseudomonas aeruginosa (ATCC 9027), Pseudomonas fluorescens (ATCC 13525) and gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Bacillus subtilis (ATCC 6633), August 2022⎪Vol.

Techniques: Microscopy, Staining, Modification

Journal: Journal of microbiology and biotechnology

Article Title: Inhibition of Pathogenic Bacteria and Fungi by Natural Phenoxazinone from Octopus Ommochrome Pigments.

doi: 10.4014/jmb.2206.06043

Figure Lengend Snippet: Fig. 7. Cellular turbidity inhibition (OD600) after 24 h of treatment with ommochromes at concentrations of 0.31 to 10 mg/ml against S. aureus and E. coli using chloramphenicol (100 μg/ml) as a positive control. Results represent the mean ± SD of at least 3 experiments, in triplicate. The different letters indicate significant differences (p < 0.05) in each microorganism.

Article Snippet: For the antibacterial evaluation, the gram-negative pathogenic bacteria Escherichia coli (ATCC 25922), Salmonella Typhimurium (ATCC 14028), Pseudomonas aeruginosa (ATCC 9027), Pseudomonas fluorescens (ATCC 13525) and gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Bacillus subtilis (ATCC 6633), August 2022⎪Vol.

Techniques: Inhibition, Positive Control

Journal: Journal of microbiology and biotechnology

Article Title: Inhibition of Pathogenic Bacteria and Fungi by Natural Phenoxazinone from Octopus Ommochrome Pigments.

doi: 10.4014/jmb.2206.06043

Figure Lengend Snippet: Fig. 9. Microbial growth (%) of bacteria treated with ommochromes (0.31-10 mg/ml) at 24 h, determined by the resazurin method for (A) S. aureus, (B) B. subtilis, (C) E. coli, (D) S. Typhimurium, and (E) P. aeruginosa, using chloramphenicol (100 μg/ml) as a positive control. Results represent the mean ± standard deviation of at least 3 experiments in triplicate. The different letters indicate significant differences (p < 0.05).

Article Snippet: For the antibacterial evaluation, the gram-negative pathogenic bacteria Escherichia coli (ATCC 25922), Salmonella Typhimurium (ATCC 14028), Pseudomonas aeruginosa (ATCC 9027), Pseudomonas fluorescens (ATCC 13525) and gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Bacillus subtilis (ATCC 6633), August 2022⎪Vol.

Techniques: Bacteria, Positive Control, Standard Deviation

Journal: Environment international

Article Title: Untouchability of natural spa waters: Perspectives for treatments within a personalized water safety plan.

doi: 10.1016/j.envint.2019.105095

Figure Lengend Snippet: Fig. 2. Bacteriostatic activity in natural SPA waters with different abiotic components and a spring drinkable water with low mi- neral content (BLM): E. coli survival after 24 h of incubation at 37 °C. As additional controls were used 0.9% Saline Solution (SS) and Phosphate Buffer Saline (PBS). Values of CFUs are normalized on the t0 value and data are expressed as percentage of survival. Mean value and standard deviation of ex- periments in triplicate.

Article Snippet: Non-pathogenic Escherichia coli (ATCC 35218) strain was used in this study.

Techniques: Activity Assay, Incubation, Saline, Standard Deviation

Journal: Environment international

Article Title: Untouchability of natural spa waters: Perspectives for treatments within a personalized water safety plan.

doi: 10.1016/j.envint.2019.105095

Figure Lengend Snippet: Fig. 3. Increment of the native antibacterial activity by TiO2 treatment. Experimental test with high mi- neral content water (W3, sulphate bicarbonate water containing alkaline hearth metals) after contamina- tion with E. coli and treatment with TiO2 nano- particles. Data were expressed as survival percen- tages of E. coli bacteria after exposition to dark, ultraviolet (UV) and white colour light at visible wavelengths. Data collected at different times of exposition: blue bars before TiO2 exposition, orange bars at 1 h and grey bars after 5 h of TiO2 exposition. (For interpretation of the references to colour in this figure legend, the reader is referred to the web ver- sion of this article.)

Article Snippet: Non-pathogenic Escherichia coli (ATCC 35218) strain was used in this study.

Techniques: Activity Assay, Bacteria

Journal: Environment international

Article Title: Untouchability of natural spa waters: Perspectives for treatments within a personalized water safety plan.

doi: 10.1016/j.envint.2019.105095

Figure Lengend Snippet: Fig. 4. Experimental test of low mineral content water contaminated with E. coli and treatment with (blue circle) or without TiO2 nanoparticles (red circle). Data were expressed as means of CFU x 102 count on agar plates incubation after 120 min exposure (T120) at several wavelengths of the visible spectrum. Starting E. coli concentration at T0: < 105 (A) and > 105 (B). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Non-pathogenic Escherichia coli (ATCC 35218) strain was used in this study.

Techniques: Incubation, Concentration Assay

Journal: Environmental science & technology

Article Title: Short-term inactivation rates of selected Gram-positive and Gram-negative bacteria attached to metal oxide mineral surfaces: role of solution and surface chemistry.

doi: 10.1021/es4003923

Figure Lengend Snippet: Figure 1. Inactivation rates based on Live/Dead BacLight viability measurements for different solution ionic strengths (1, 10, and 100 mM) of (a) KCl and (b) CaCl2 for E. coli D21f2, E. coli O157:H7, and E. faecalis adhered onto Fe2O3. The error bars indicate standard deviations.

Article Snippet: Two Gram negative bacteria, nonpathogenic E. coli D21f2 and pathogenic E. coli O157:H7 (ATCC 700927), and a Gram positive organism, pathogenic E. faecalis (ATCC 29212) were used as the test bacteria.

Techniques:

Journal: Environmental science & technology

Article Title: Short-term inactivation rates of selected Gram-positive and Gram-negative bacteria attached to metal oxide mineral surfaces: role of solution and surface chemistry.

doi: 10.1021/es4003923

Figure Lengend Snippet: Figure 2. (a) Extent of bacterial attachment (EA) and (b) inactivation rates (ri) for E. coli D21f2, E. coli O157:H7, and E. faecalis adhered to different surfaces (silica, Fe2O3, and Al2O3) in 10 mM KCl. The error bars indicate standard deviation. An asterisk indicates a statistically significant difference in inactivation rates when compared to the silica treatment (determined using one-way ANOVA, p < 0.005).

Article Snippet: Two Gram negative bacteria, nonpathogenic E. coli D21f2 and pathogenic E. coli O157:H7 (ATCC 700927), and a Gram positive organism, pathogenic E. faecalis (ATCC 29212) were used as the test bacteria.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Simple and Versatile Turbidimetric Monitoring of Bacterial Growth in Liquid Cultures Using a Customized 3D Printed Culture Tube Holder and a Miniaturized Spectrophotometer: Application to Facultative and Strictly Anaerobic Bacteria

doi: 10.3389/fmicb.2016.01381

Figure Lengend Snippet: Growth curves of Escherichia coli obtained by measuring the OD at 600 nm in the miniaturized setup (optical path of ∼16 mm) (triangles), and the benchtop UV-Vis spectrophotometer (optical path of 10 mm) (circles). Results are means and standard deviation from three cultures.

Article Snippet: The pathogenic, facultative anaerobic bacteria Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 were obtained from the General Hospital of Santo António – Centro Hospitalar do Porto (Porto, Portugal).

Techniques: Spectrophotometry, Standard Deviation